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transposed vector meaning in Chinese

转置向量

Examples

  1. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ) , and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee . the plasmid pfb 1 - cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies , which was recombinant baculovirus shuttle vector containing hcv structural gene , named bac - cee
    本实验用pcr扩增hcv结构区基因,克隆到杆状病毒表达载体pfastbacl中,构建成重组转座载体pfb1 - cee ,转化dh10bac大肠杆菌感受态细胞,筛选阳性菌落,抽提大分子质粒dna ,获得含hcv结构区基因的重组杆状病毒穿梭载体bac - cee ,脂质体介导转染sf9昆虫细胞,出现细胞病变后,收集含有重组杆状病毒颗粒的培养上消,重新感染sf9细胞,收集sf9细胞,进行12 . 5 sds -聚丙烯酰胺凝胶电泳,可见表达的蛋白条带。

Related Words

  1. transposing
  2. transpose
  3. transposed tooth
  4. transposed equation
  5. transposed twill
  6. transposed dyadic
  7. transpose file
  8. transpose chain
  9. transmit transpose
  10. transpose command
  11. transposed transmission line
  12. transposed twill
  13. transposedmatrix
  14. transposer
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