transposed vector meaning in Chinese
转置向量
Examples
- In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ) , and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee . the plasmid pfb 1 - cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies , which was recombinant baculovirus shuttle vector containing hcv structural gene , named bac - cee
本实验用pcr扩增hcv结构区基因，克隆到杆状病毒表达载体pfastbacl中，构建成重组转座载体pfb1 - cee ，转化dh10bac大肠杆菌感受态细胞，筛选阳性菌落，抽提大分子质粒dna ，获得含hcv结构区基因的重组杆状病毒穿梭载体bac - cee ，脂质体介导转染sf9昆虫细胞，出现细胞病变后，收集含有重组杆状病毒颗粒的培养上消，重新感染sf9细胞，收集sf9细胞，进行12 . 5 sds -聚丙烯酰胺凝胶电泳，可见表达的蛋白条带。